亜硝酸ナトリウムを用いるズルチンの新けい光分析法(第1報) : 定量法の開発とその食品分析への応用

概要

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A new fluorometric determination of dulcin (4-ethoxyphenylurea) was established as follows : To 1 ml of dulcin solution in a test tube with a stopper is added 0.4 ml of 1N HCl, and the test tube is heated in a boiling water bath for 10 minutes. After cooling to room temperature, 0.5 ml of 10% NaNO_2 solution is added and the solution is allowed to stand for 30 minutes at room temperature. Then, 0.5 ml of 6% NaOH solution is added and the solution is allowed to stand for 40 minutes at room temperature. Subsequently, the solution is extracted with 10 ml of CHCl_3 by an automatic shaker for 5 minutes. The CHCl_3 phase gives a fluorescence spectrum with an excitation maximum at 354 mμ and an emission maximum at 446 mμ. A straight line relationship between dulcin concentration and fluorescence intensity was obtained within the range of 1-8 μg/ml in CHCl_3. This method was successfully applied to the determination of dulcin in some foods.
公益社団法人日本薬学会の論文
1969-06-25