アンモニアの吸光光度定量法(第2報) : グアヤコールを用いたアンモニアの新吸光光度定量法

概要

論文の詳細を見る
A new colorimetric method for the determination of ammonia with guaiacol and sodium hypochlorite was studied and a recommended procedure was proposed. This method gave remarkably lower absorbance of blank and better reproducibility than the previously reported o-phenylphenol method. With considerations on the occurrence of side reaction and the reaction rate, suitable concentrations of hypochlorite and nitroprusside were determined. Almost all the variables influencing the color reaction were examined. Coefficient of variance at 20 μg/ml ammonium sulfate solution and apparent molar extinction coefficient (based on N atom) at 680 mμ were found to be ca. 2.3% and 1.92×10^4,respectively. These values indicate sufficient sensitivity for estimating the quantity of ammonia and correspond to absorbance of 1.36 per μg・N/ml of the final colored solution. The probable reasons for the better reproducibility and low blank value were also briefly discussed.
1969-06-25