アンモニアの吸光光度定量法(第3報) : m-クレゾールを用いた新吸光光度定量法

概要

論文の詳細を見る
Use of m-cresol improved the reproducibility of data and lowering of blank value from any other method for the determination of ammonia through indophenol formation. The present method demonstrated a straight linearity between the concentration of ammonium sulfate, from 0 to 30 μg/ml, and the absorbance. Apparent molar extinction coefficient on the basis of N atom and coefficient of variance at 20 μg/ml ammonium sulfate were 2.48 x 10^4 and 1.60%, respectively. Absorbance of blank solution was satisfactorily as low as 0.012±0.003. Reagents used remained stable only for three days. New imformations concerning reaction mechanism were also obtained. First, the absorbance was dependent not only on the order or the interval of the addition of hypochlorite and m-cresol but also concurrently on the concentration of the former (Fig. 2). Secondly, the decrease in the concentration of monochloramine, which had formed at the early stage of the reaction, resulted in lowering of absorbance that declined exponentially as a function of addition interval of the two reagents.
公益社団法人日本薬学会の論文
1969-11-25