リソゾーム膜不安定化剤による線維芽細胞からのMucopolysaccharidaseの放出に及ぼすD-Penicillamineの作用

概要

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The effect of D-penicillamine on release of mucopolysaccharidases (N-acetyl-β-glucosaminidase and β-glucuronidase) by lysosomal labilizers was tested using cultured fibroblasts derived from rat carrageenin granuloma. The release of N-acetyl-β-glucosaminidase and β-glucuronidase into the culture medium was stimulated by treatment with Ca-EDTA (1 mM) or vitamin A (10 μg/ml) by which the fibroblasts remain morphologically intact. On the other hand, the release of both enzymes into the culture medium was inhibited by treatment with D-penicillamine (over 1 mM). However, when 0.5 mM D-penicillamine, which shows no effect on the release of mucopolysaccharidases, was added simultaneously with Ca-EDTA or vitamin A, release of both enzymes was considerablly inhibited. These results are discussed in relation to the mode of action of D-penicillamine as an antirheumatic drug.
社団法人日本薬学会の論文
1977-10-25