フェノール誘導体のけい光分析(第5報)塩酸フェニレフリンのけい光定量

概要

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A fluorometric method has been investigated for the determination of phenylephrine hydrochloride (I). I was converted with HCHO into a condensed substance (II) which was oxidized by K_3Fe(CN)_6 in 1N NaOH solution to a fluorescent substance (III). Based on these reactions, a fluorometric determination of I was established. Chemical structures of II and III were found to be respectively 4,6-dihydroxy-2-methyl-1,2,3,4-tetrahydro-isoquinoline and 6-hydroxy-2-methylisoquinolinium chloride which is in an amphoteric ion form in an alkaline solution. The anlytical procedure is as follows : To 1 ml of a solution of I (2.5 μg/ml), 1 ml of 20% HCHO solution is added, the mixed solution is allowed to stand for 15 min at room temperature, and 5 ml of 1N NaOH solution and 1ml of 0.25% K_3Fe (CN)_6 solution are added to this solution. After standing for 45 min at room temperature, 1 ml of 0.15% ascorbic acid solution is added, and the whole is diluted to 20 ml with methanol. The fluorescence intensity of the solution is measured at 450 mμ, exciting at 370 mμ.
公益社団法人日本薬学会の論文
1975-08-25