リボフラビン存在下, 大腸菌転移RNAの光増感失活に関する研究(第2報)光増感失活におよぼすアデニンの影響

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Previous studies on the photodynamic inactivation of various biological substances in the presence of riboflavin have shown that photoinactivation of yeast alcohol dyhydrogenase (EC 1.1.1.1) and Bacillus subtilis transforming DNA was greatly accelerated by the addition of adenine. Attempts were made to examine whether a similar phenomenon would also be observed in the riboflavin-sensitized photoinactivation of Escherichia coli tRNA and to elucidate the basic mechanism involved in the reaction. The riboflavin-sensitized photoinactivation of tRNA is remarkably accelerated by the addition of adenine under aerobic conditions. Adenine can also promote the photodynamic inactivation of tRNA with FAD, FMN, and lumiflavin, while the reaction with lumichrome, Methylene Blue, thiopyronine, and Eosin Yellow is not affected by adenine. The adenine derivatives such as adenine 1-N-oxide, AMP, ADP, and NAD also accelerate the riboflavin-sensitized photoinactivation of tRNA, whereas guanine, cytosine, thymine, uracil, and adenosine do not accelerate the photoinactivation significantly. The enhancement of the riboflavin-sensitized photoinactivation of tRNA by adenine may be ascribed to the promotion of the destruction of guanine residues in tRNA and to the acceleration of changes of base stacking and the disruption of base pairs, which seems to result from the destruction of guanine residues in tRNA. The promotion of the photodynamic degradation of 4-thiouracil residues may also be responsible for the enhancement of the inactivation of tRNA, since disappearance of its characteristic absorption maximum near 335mμ was promoted by adenine. The mechanism through which adenine causes the enhancement of the riboflavin-sensitized photoinactivation of tRNA is discussed.
社団法人日本薬学会の論文
1973-06-25

リボフラビン存在下, 大腸菌転移RNAの光増感失活に関する研究(第2報)光増感失活におよぼすアデニンの影響

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