リボフラビン存在下, 大腸菌転移RNAの光増感失活に関する研究(第1報)転移RNAの光増感失活に伴う構成塩基類の化学的変化

概要

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Irradiation of Escherichia coli transfer RNA (tRNA) with visible light in the presence of oxygen and riboflavin led to the loss of its amino acid-accepting activity. In an attempt to elucidate the basic mechanism involved in the riboflavin-sensitized photoinactivation of tRNA, changes in base compositions of tRNA as a result of photodynamic treatment were examined. The photodynamic inactivation of tRNA was found to be ascribable to the selective destruction of guanine residues in tRNA and to the changes in its tertiary structure, which involved decrease in the degree of base stacking and disruption of base pairs. The modification of 4-thiouracil residues in tRNA may also be involved in the riboflavin-sensitized photoinactivation, since its characteristic absorption maximum near 335 mμ disappeared by the photodynamic treatment. Though guanine and adenine derivatives are susceptible to the riboflavin-sensitized photodegradation, the adenine residues in tRNA were unaffected even when amino acid-accepting activity of tRNA was almost completely lost.
社団法人日本薬学会の論文
1973-04-25