D-Arabinosoneの酵素化学的研究(第7報) : ブタ肝臓中のD-Arabinosone自動酸化抑制物質

概要

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The substances preventing the autoxidation of D-arabinosone were extracted from swine liver and purified by ammonium sulfate fractionation, acetone fractionation, acid precipitation, DEAE-cellulose column chromatography, and carboxymethylcellulose column chromatography. One of them was obtained in homogeneous form as judged by polyacrylamide gel electrophoresis and was designated as "antioxidant II." It gave positive color reactions for protein, and its absorption spectrum was typical of protein. Its molecular weight as determined by gel filtration was in the range of 27000 to 29000. Antioxidant II is acid-and alkali-labile, and is inactivated above 60°. It also prevented the autoxidation of L-arabinosone, D-xylosone, D-glucosone, and L-ascorbic acid. Although yeast alcohol dehydrogenase and L-cysteine at relatively high concentrations prevented the autoxidation of D-arabinosone, other proteins, sulfhydryl compounds, amino acids, and α-tocopherol showed no preventing effect on the autoxidation. These results suggested that antioxidant II is quite specific in its antioxidative property.
社団法人日本薬学会の論文
1971-09-25