Dihydrothiamineの酵素的酸化反応 : (I)Tyrosinaseによる酸化反応

概要

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In the present report, the experiments were performed to prove that dihydrothiamine (DHB_1), assumed as the precursor of thiamine biosynthesis, was oxidatively converted to thiamine using the partially purified potato tyrosinase. The results indicated that the tyrosinase-catechol system catalyzed the oxidation of DHB_1 to thiamine to the extent of 20% only when both tyrosinase and catechol were added. Some of other phenolic compounds, such as guaiacol and L-tyrosine was less useful. This enzymatic oxidation showed rather sharp pH optimum between 6 to 7,and the results obtained at optimum pH showed that the amount of thiamine produced was proportional to catechol concentration and tyrosinase activity within a limited range. The possibility that DHB_1 may be a precursor of thiamine biosynthesis in nature was discussed.
日本ビタミン学会の論文
1962-07-25