デオキシリボ核酸(DNA)に対する6-Mercaptopurineの光力学作用

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We examined the photodynamic action of 6-mercaptopurine on calf thymus DNA and the mechanisms involved in this reaction. When the aqueous mixture of 6-mercapto-purine and DNA was irradiated by light from a 290 nm distance, the melting point and viscosity of DNA decreased, the alkaline sucrose density gradient centrifugation pattern of DNA shifted towards the less dense side, and malonaldehyde was produced in the reaction mixture. These changes were inhibited when the reaction mixture was degassed, or when a quencher for singlet oxygen or excited triplets was added to the reaction mixture. We concluded that single-chain scission of the DNA molecule occurred as a result of the photodynamic action of 6-mercaptopurine, and that singlet oxygen produced in the reaction mixture played an important role in the photosensitization of DNA.
社団法人日本薬学会の論文
1980-03-25