テオキシリボ核酸(DNA)に対するアントラキノン誘導体の光力学作用 : DNA鎖の切断

概要

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Examinations were made on the photodynamic action of anthraquinone derivatives on calf thymus DNA and also on the mechanism involved in this reaction. When the aqueous mixture of DNA and anthraquinone derivatives was irradiated by light above 290 nm, melting point and viscosity of DNA decreased in the order of 2-anthraquinonesulfonate, 2-anthraquinonecarboxylic acid, and 1-anthraquinonesulfonate. Such effect was not seen in 1,5- and 1,8-anthraquinonedisulfonate. The degree of the photodynamic action of anthraquinones on DNA paralleled the quantity of hydrogen peroxide produced by the photoirradiation of aqueous solution of anthraquinone derivatives. Results of the "i-assay" and sucrose density gradient centrifugation experiments indicated that scission of the DNA chain took place by the photodynamic action of anthraquinone derivatives. Examination of the reaction mechanism revealed that hydroxy radical produced in the aqueous solution of anthraquinone derivatives during photoirradiation played an important role in the photosensitization reaction of DNA.
公益社団法人日本薬学会の論文
1978-07-25