ラット腎髄質組織からのProstaglandin E遊離に対するTheophyllineの作用

概要

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Effect of theophylline on intrarenal prostaglandin (PG) content and the PGE release from renal medulla was studied by using male Wistar rats. PG was assayed biologically by use of rat stomach fundus. The PGE content in the renal medulla increased 2 hours after oral administration of theophylline (50 mg/kg p.o.). The PGE release from renal medullary slices into Krebs-Ringer bicarbonate buffer was increased by the administration of theophylline both in vivo (50 mg/kg) and in vitro (10^<-5>M). The effect of theophylline in vitro on PGE release from renal medulla into the Krebs-Ringer bicarbonate buffer was completely inhibited by the addition of indomethacin (10^<-5>M) or aspirin (10^<-3>M). The urinary cyclic AMP excretion and urine volume were increased by the oral administration of theophylline. On the other hand, addition of dibutyryl cyclic AMP (10^<-2>M) increased the PGE release from renal medullary tissue. These results suggest that the increase of renal PGE induced by theophylline plays an important role in the mechanism of theophylline diuresis. Moreover, it is thought that intrarenal cyclic AMP may participate, at least in part, in the stimulation of PGE release from renal medullary tissue by theophylline.
公益社団法人日本薬学会の論文
1981-10-25