ラット骨格筋ミトコンドリア酵素活性に及ぼすSerotonin局所投与の影響およびこれに対する抗炎症薬の作用

概要

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Influence of local administration of serotonin on mitochondrial enzyme activities of rat skeletal muscle and action of anti-inflammatory drugs on it were studied. Two hundred μg of serotonin was injected into left gastrocnemius muscle. The right muscle was used for the control. The muscle was isolated 6 hr after serotonin injection and homogenized with 0.1 M Tris-HCl buffer containing 0.25 M sucrose (pH 7.4). The supernatant at 600×g of the homogenate was used as the enzyme source. Succinic dehydrogenase, malic dehydrogenase, lactic dehydrogenase, and ascorbate oxidase activities were determined in isotonic medium. Succinic dehydrogenase activity of the serotonin-injected muscle was about 3.2-fold of that of the control, but the other enzyme activities were not significantly changed. Aspirin, hydrocortisone, and indomethacin inhibited the increased activity of succinic dehydrogenase. Malic dehydrogenase and ascorbate oxidase activities of control and serotonin-injected muscle decreased by the administration of aspirin or hydrocortisone. Lactic dehydrogenase activity of both muscles was inhibited by the administration of aspirin, and increased by the administration of indomethacin.
1976-02-25