Determination of Urinary Prostaglandin E_2 by the Use of Prostaglandin F_<2α> Antiserum and Sodium Borohydride

概要

論文の詳細を見る
A radioimmunoassay (RIA) for prostaglandin E_2 (PGE_2) based on the use of commercial PGF_<2α> antiserum is described. PGE was extracted from human urine and separated by silicic acid column chromatography, then PGE was converted to PGF by the use of sodium borohydride (NaBH_4), and the formed PGF_<2α> was determined radioimmunologically with commercial PGF_<2α> antiserum. There was a good correlation (y=0.841x+0.518,r=0.912) between added standard PGE_2 and measured PGE_2 levels in human urine. Moreover, a good correlation (y=1.46x+0.569,r=0.952) was also found between the PGE_2 levels in the urine obtained by means of the bioassay previously reported and those obtained by the proposed method. Therefore, this method has high accuracy and good reproducibility. Furthermore, it is also an advantage that radioimmunological assays of PGF_<2α> and PGE_2 in biological samples can be performed separately with only one antiserum by this method.
公益社団法人日本薬学会の論文
1983-10-25