水素細菌Alcaligenes hydrogenophilus の水素資化能の異種微生物への付与

概要

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The plasmid genes encoding the ability to grow chemolithoautotrophically with H_2 and CO_2 from a H_2-oxidizing bacterium Alcaligenes hydrogenophilus were in vivo cloned using the broad host range Inc P1 R-plasmid R68.45. The genes of H_2-oxidation enzymes were expressed by a novel alternative σ^<54>-like factor encoded on the chromosome. The σ^<54>-like factor was also in vivo cloned using R68.45. Both R68.45-primes, one carrying plasmid genes and the other carrying chromosome genes, were in vivo recombinated and a recombinant plasmid carrying both genes from plasmid and chromosome was obtained. The conjugal transfer of chemolithoautotrophically growing ability was carried out using the resulting recombinant plasmid. Seventeen bacterial strains, including useful material-producing bacteria, grew up to be able to grow with H_2 and CO_2 as the H_2-oxidizing bacteria. Some patent strains registered for the production of antibiotics were ascertained to produce some products which inhibited the growth of the testingbacteria, under not only heterotrophic conditions but also chemolithoautotrophilc conditions. The results obtained in our studies will be available in the future research for the production of useful material from CO_2.
公益社団法人日本薬学会の論文
1994-02-25