水銀(II)-チオテノイルトリフルオルアセトン錯体を用いたチオシアン酸イオンの吸光光度定量法

概要

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It was found that color intensity of the complex between mercury (II) and thiothenoyltrifluoroacetone (STTA) is suppressed in the presence of thiocyanate ion. This suppression was applied to the determination of a small amount of thiocyanate ion in the solution acidified by sulfuric acid. The proposed method covers a concentration range of 5-60 μg of thiocyanate ion in 10 ml solution. The coefficient of variation was 2.1,2.0,and 1.1% for 10,30,and 50 μg of thiocyanate ion, respectively. The analytical procedure proposed is as follows. Eight milliliters of a sample solution containing 5-60 μg thiocyanate ion and 2 ml of 10 N sulfuric acid are placed in a test tube with a ground-glass stopper, 10 ml of mercury (II)-STTA complex solution containing 40 μg mercury (II) in carbon tetrachloride (10 ml) is added, and the mixture is shaken vigorously for 2 min. The organic layer is separated, washed successively, with 10 ml each of water and pH 11.5 washing solution, and dried over anhydrous sodium sulfate. The absorbance of mercury (II)-STTA complex in the organic layer is measured at 360 nm against carbon tetrachloride. Examination of the effect of co-existing anions showed that sulfide, iodide, and iodate interfered even in one-tenth the amount of thiocyanate ion. The proposed method was applied to the determination of thiocyanate ion in human saliva.
公益社団法人日本薬学会の論文
1972-08-31