Assay for Tryptophan Hydroxylase Activity in Rat Brain by High-Performance Liquid Chromatography with Fluorescence Detection
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概要
- 論文の詳細を見る
A highly sensitive and selective liquid chromatographic (HPLC) method with post-column fluorescence detection has been developed for the determination of tryptophan 5-monooxygenase activity in rat brain tissue homogenate. 5-Hydroxytryptophan, formed enzymatically from tryptophan (incubation time, 20 min), is extracted with perchloric acid and determined by HPLC. Detection is performed fluorometrically after post-column derivatization by a reaction with benzylamine in the presence of potassium hexacyanoferrate (III). The lower limit of detection for 5-hydroxytryptophan formed enzymatically is 100 fmol at a signal-to-noise ratio of three.
- 社団法人日本薬学会の論文
- 1996-05-15
著者
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Ishida Junichi
大阪大学 保健学科医用物理学
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Ishida Junichi
Faculty Of Pharmaceutical Sciences Fukuoka University
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Yoshitake Takashi
Department Of Neuroscience Karolinska Institutet
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YAMAGUCHI Masatoshi
Faculty of Pharmaceutical Sciences, Fukuoka University
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YOSHITAKE Takashi
Chemicals Evaluation and Research Institute
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IIZUKA Ryuji
Chemicals Evaluation and Research Institute
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NAKAMURA Masaru
Faculty of Pharmaceutical Sciences, Fukuoka University
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Iizuka Ryuji
Chemical Biotesting Center Chemical Inspection And Testing Institute
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Takata Yutaka
Department Of Gastroenterology National Kyushu Cancer Center
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Yakabe Tomohiro
Faculty Of Pharmaceutical Sciences Fukuoka University
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Yakabe Tomohiro
Department Of Legal Medicine Shimane Medical University
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Yamaguchi Masatoshi
Faculty Of Pharmaceutical Sciences Fukuoka University
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Ishida J
Faculty Of Pharmaceutical Sciences Fukuoka University
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Yoshitake Takashi
Chemical Biotesting Center Chemical Inspection And Testing Institute
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Nakamura Masaru
Faculty Of Pharmaceutical Sciences Fukuoka University
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Nakamura M
Faculty Of Pharmaceutical Sciences Fukuoka University
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Nakamura Masaru
Faculty Of Medicine Teikyo University
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Nakamura Masaru
Departments of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University
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