Specificities of Five Kinds of Antisera Produced against Crude Drugs, Pinellia Tuber, Hoelen, Glycyrrhizae Radix, Trichosanthes Root and Panax Ginseng
スポンサーリンク
概要
- 論文の詳細を見る
Five kinds of rabbit antisera produced against five kinds of crude drugs, Pinella Tuber, Glycyrrhizae Radix (GR), Trichosanthes Root, Hoelen, and Panax Ginseng, and five kinds of extracts of the same crude drugs were prepared. Specificity of each antiserum was demonstrated by two immunological methods for analyses, the selected antibody enzyme immunoassay (SAEIA) and western blotting, and five crude drug extracts as specimens. Each crude drug extract contained characteristic antigen specific to the corresponding antiserum. Characteristic antigens were suggested as protein components. Characteristic antigen of the crude drug GR was separated from GR extract by three chromatographic procedures and a protein component was separated. The separated protein was successfully applied to develop a SAEIA method applicable for specific assays of both the separated protein and GR extract.
- 公益社団法人日本薬学会の論文
- 1996-03-15
著者
-
BAI Gang
Faculty of Pharmaceutical Sciences, Nagasaki University
-
FUJIWARA Kunio
Faculty of Pharmaceutical Sciences, Nagasaki University
-
KITAGAWA Tsunehiro
Faculty of Pharmaceutical Sciences, Nagasaki University
-
Bai Gang
Faculty Of Pharmaceutical Sciences Nagasaki University
-
Fujiwara K
Faculty Of Pharmaceutical Sciences Nagasaki University
-
Kitagawa T
Kobe Gakuin Univ. Kobe Jpn
-
Fujiwara Kunio
Faculty Of Pharmaceutical Sciences Nagasaki University
-
Kitagawa Tsunehiro
Faculty Of Pharmaceutical Sciences Nagasaki University
-
Kitagawa Tsunehiro
Faculty of Pharmaceutical Sciences and High Technology Research Center, Kobe Gakuin University
関連論文
- Enzyme Immunoassay for a Characteristic Protein in the Animal Crude Drug Lumbricus
- Application of a Licorice Root Specific Protein to a General Method for the Assay of Licorice Root Components in Traditional Chinese Medicines
- A General Enzyme Immunoassay for the licorice Root Component Contained in Traditional Chinese Medicines
- A New Method for the Detection and Quantitative Measurement of the Contents of Trichosanthes Root Component Composing Chinese Traditional Medicines
- DETECTION OF RESIDUAL PENICILLIN IN MILK BY SENSITIVE ENZYME IMMUNOASSAY
- Amino Acids and Peptides. XXVII. Solid Phase Synthesis of Fibrinogen-Related Peptides with Disulfide Bond Formed on Solid Support
- Glutaraldehyde (GA)-Hapten Adducts, but without a Carrier Protein, for Use in a Specificity Study on an Antibody against a GA-Conjugated Hapten Compound: Histamine Monoclonal Antibody (AHA-2) as a Model
- ENZYME IMMUNOASSAY OF RABBIT IMMUNOGLOBULIN G AND ITS APPLICATION
- Synthesis and Root Growth-Inhibitory Activity of 2- and 3-(Haloacetylamino)-3-(2-furyl)propanoic Acids
- Mithramycin Represses MDR1 Gene Expression in Vitro, Modulating Multidrug Resistance
- Two Novel C-Glycosides of Aureolic Acid Repress Transcription of the MDR1 Gene
- Preparation of Monoclonal Antibodies against N-(γ-Maleimidobutyryloxy)succinimide (GMBS)-Conjugated Acetylspermine,and Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for N^1,N^-Diacetylspermine
- Development and Application of a Sandwich Enzyme Immunoassay for Glycyrrhizae Radix Protein (GRP) Using Monoclonal Antibodies
- Specificities of Five Kinds of Antisera Produced against Crude Drugs, Pinellia Tuber, Hoelen, Glycyrrhizae Radix, Trichosanthes Root and Panax Ginseng
- Determination of Urinary Acetylpolyanines by a Monclonal Antiboby-Based Enzyme-Linked Immunosorbent Assay (ELISA)
- ENZYME IMMUNOASSAY OF NEOCARZINOSTATIN USING β-GALACTOSIDASE AS LABEL
- STUDIES ON IMMUNOGLOBULINS : PURIFICATION OF ANTI-HAPTEN ANTIBODY AND DEVELOPMENT OF HIGH SENSITIVE ENZYME IMMUNOASSAY FOR HUMAN IMMUNOGLOBULIN E
- ENZYME IMMUNOASSAYS OF SEVERAL ANTIBIOTICS AND THEIR APPLICATION
- Histamine Monoclonal Antibody for Brain Immunocytochemistry
- PYROGLUTAMYL PEPTIDASE FROM CHICKEN LIVER : PURIFICATION AND SOME PROPERTIES
- INVESTIGATIONS ON PRODUCTIONS OF THE SPECIFIC ANTISERA TO A HAPTEN AND TO A CARRIER PROTEIN ON A NEW METHOD FOR PREPARATION OF ANTISERA TO HAPTENS