ENZYME IMMUNOASSAY OF NEOCARZINOSTATIN USING β-GALACTOSIDASE AS LABEL
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概要
- 論文の詳細を見る
A novel convenient procedure was introduced for enzyme labelling of neocarzinostatin (NCS) with β-D-galactosidase using a new hetero-bifunctional reagent N-(γ-maleimidobutyryloxy) succinimide (GMBS). With the enzyme labelled NCS and a rabbit anti-NCS serum which was elicited in a rabbit immunized with NCS in complete Freund's adjuvant, a highly sensitive enzyme immunoassay which can quantify a femto mol order NCS was developed. Accuracy and precision of the assay as well as a comparison with the immunodiffussion method were studied with satisfactory results. This enzyme immunoassay of NCS was applicable to monitoring serum NCS levels of patients in clinical treatment of the antitumor agent.
- 公益社団法人日本薬学会の論文
著者
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TSUNODA TSUKASA
Department of Gastroenterological Surgery, Kawasaki Medical School
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KITAGAWA Tsunehiro
Faculty of Pharmaceutical Sciences, Nagasaki University
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Tsunoda Tsukasa
Department Of The Second Surgery School Of Medicine Nagasaki University
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Tsunoda Tsukasa
Department Of Gastroenterological Surgery
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TANIMORI HIDEAKI
Faculty of Pharmaceutical Sciences, Nagasaki University
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Tanimori Hideaki
Faculty Of Pharmaceutical Sciences Nagasaki University
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TSUCHIYA RYOICHI
Department of the Second Surgery, School of Medicine, Nagasaki University
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Tsuchiya Ryoichi
Department Of The Second Surgery School Of Medicine Nagasaki University
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Kitagawa Tsunehiro
Faculty Of Pharmaceutical Sciences Nagasaki University
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Kitagawa Tsunehiro
Faculty of Pharmaceutical Sciences and High Technology Research Center, Kobe Gakuin University
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