A Reliable Method for Intratracheal Instillation of Materials to the Entire Lung in Rats
スポンサーリンク
概要
- 論文の詳細を見る
A novel method for intratracheal instillation of rats is described by which an operator can confidently administer a liquid sample into the tracheal lumen, with direct observation of the entrance to the trachea. To confirm its efficacy, indigo carmine was administered with or without air to investigate optimal conditions for exposure of the whole lung. After administration of 0.5 ml of indigo carmine and an equal volume of air simultaneously by intratracheal instillation, sections through the whole lung demonstrated positive staining with the dye. For histological examination, 0.5 ml of saline containing iron oxide followed by an equal volume of air was administered intratracheally. Iron particles were observed in the wall of bronchioles and alveolar macrophages of whole lung. The method allows reliable intratracheal administration to large numbers of rats within a short period and should, therefore, prove useful for assays of toxicity in the respiratory tract.
- 日本毒性病理学会の論文
- 2006-06-01
著者
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MORI Toshio
RI Center, Nara Medical University
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Sakamoto Akika
Division of Pathology, Bozo Research Center Inc.
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Mitsui Masayuki
Mitsui Consulting
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Tsutsumi Masahiro
RI Center, Nara Medical University
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TSUNODA TSUKASA
Department of Gastroenterological Surgery, Kawasaki Medical School
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KUSUOKA Osamu
Department of Oncological Pathology, Cancer Center, Nara Medical University
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Kusuoka Osamu
Department Of Molecular Pathology Nara Medical University
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OKA Yasuo
Department of Materials Physics, Faculty of Engineering Science, Osaka University
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Tsutsumi Masahiro
Ri Center Nara Medical University
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Oka Yasuo
Department Of Surgery Division Of Gastroenterology Kawasaki Medical University
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Oka Yasuo
Department Of Digestive Surgery Kawasaki Medical School
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Kitahashi Tsukasa
Ri Center Nara Medical University
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Tsunoda Tsukasa
Department Of Gastroenterological Surgery
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Tsunoda Tsukasa
Department Of Surgery Division Of Gastroenterology Kawasaki Medical University
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MITSUI Masayuki
Department of Molecular Pathology, Nara Medical University
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SAKAMOTO Akika
RI Center, Nara Medical University
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Mori Toshio
Ri Center Nara Medical. Univ.
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Mitsui Masayuki
Department Of Molecular Pathology Nara Medical University
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Mori Toshio
Ri Center Nara Medical Univ.
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