Infrequent Mutation of Lysophosphatidic Acid Receptor-1 Gene in Hamster Pancreatic Duct Adenocarcinomas and Established Cell Lines
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概要
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To evaluate the involvement of lysophosphatidic acid receptor-1 (LPA1) gene alteration in pancreatic carcinogenesis, we investigated mutations in the LPA1 gene in hamster pancreatic duct adenocarcinomas (PDAs) and established cell lines. Female Syrian golden hamsters received 30 mg/kg of N-nitrosobis(2-oxopropyl)amine (BOP) followed by repeated exposure to an augmentation pressure regimen consisting of a choline-deficient diet combined with DL-ethionine and then L-methionine and a further administration of 20 mg/kg BOP. A total of 10 PDAs obtained 10 weeks after beginning the experiment and three cell lines established from subcutaneously transplantable PDAs in syngeneic hamsters were examined for mutations using reverse transcription-polymerase chain reaction-single strand conformation polymorphism (RT-PCR-SSCP) analysis. A mutation was detected in only one PDA (1/10, 10%) in the form of a GGA to GTA (Gly to Val) transversion at codon 355, and no mutations were detected in the three cell lines. These results suggest that the LPA1 gene mutation may play roles in a limited fraction of BOP-induced pancreatic duct carcinogenesis in hamsters.
著者
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MORI Toshio
RI Center, Nara Medical University
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TSUJIUCHI Toshifumi
Laboratory of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and
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Furukawa Mami
Laboratory of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and
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Obo Yumi
Laboratory of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and
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Yamasaki Ayako
Laboratory of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and
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Hotta Mayuko
Laboratory of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and
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Kusunoki Chie
Laboratory of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and
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Suyama Naoko
Laboratory of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and
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Honoki Kanya
Department of Orthopedic Surgery, Nara Medical University
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Fukushima Nobuyuki
Laboratory of Molecular Neurobiology, Department of Life Science, Faculty of Science and Technology,
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Honoki Kanya
Department Of Orthopedic Surgery Nara Medical University
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Fukushima Nobuyuki
Laboratory Of Molecular Neurobiology Department Of Life Science Faculty Of Science And Engineering K
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Tsujiuchi Toshifumi
Laboratory Of Cancer Biology And Bioinfomatics Department Of Life Science Faculty Of Science And Tec
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Tsujiuchi Toshifumi
Laboratory Of Cancer Biology And Bioinformatics Department Of Life Science Faculty Of Science And En
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Mori Toshio
Ri Center Nara Medical. Univ.
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Mori Toshio
Ri Center Nara Medical Univ.
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Obo Yumi
Laboratory Of Cancer Biology And Bioinformatics Department Of Life Science Faculty Of Science And Engineering Kinki University
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