Effect of Murine Kidney Extracts on the Proliferation of Hematopoietic Progenitor Cells in Human Umbilical Cord Blood
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概要
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We examined the effect of murine kidney extract (MKE) on the clonal growth of highly purified CD34^+ hematopoietic progenitor cells from human umbilical cord blood. MKE did not affect the total number of colonies of erythroid burst-forming units (BFU-E), granulocyte-macrophage colony-forming units (CFU-GM) or granulocyte-erythroid-macrophage-megakaryocyte colony-forming units (CFU-Mix/CFU-GEMM) in a methyl-cellulose culture with exogenous recombinant human granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, interleukin-3,stem cell factor and erythropoietin. MKE significantly increased the proportion of BFU-E- or CFU-Mix-derived colonies, and suppressed the formation CFU-GM-derived colonies depending on the MKE dose. However, because of an increase in small megakaryocyte colonies derived from mature CFU-Meg MKE increased by approximately 40% the growth of megakaryocyte colony-forming units (CFU-Meg) in plasma clot culture stimulated by recombinant human thrombopoietin. Also MKE promoted an increase in hyperploid megakaryocytes, suggesting that the active factor(s) in MKE acts on the mature CFU-Meg and promotes the maturation of megakaryocytes. Gel-filtration high performance liquid chromatography of MKE showed that the promoting factor(s) in MKE was approximately 45 kDa. These results indicate that the factor(s) detected in MKE influence human hematopoiesis in vitro, especially thrombopoiesis.
- 公益社団法人日本薬学会の論文
- 2000-10-01
著者
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TAKAHASHI TSUNEO
Cell Processing Department, The Institute of Medical Science, University of Tokyo
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Takahashi T
Cell Processing Department The Institute Of Medical Science University Of Tokyo
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KASHIWAKURA Ikuo
Laboratory of Radiological Technology, Hirosaki University School of Health Sciences
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Kashiwakura Ikuo
Dep. Of Radiological Life Sciences Hirosaki Univ. Graduate School Of Health Sciences
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TAKAGI Yoshinari
Lab. Radiopharm. Sci., Hokkaido Coll. Pharm.
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MURAKAMI Miho
Laboratory of Radiopharmaceutical Sciences, Hokkaido College of Pharmacy
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HAYASE Yukitoshi
Laboratory of Radiopharmaceutical Sciences, Hokkaido College of Pharmacy
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TAKAGI Yoshinari
Laboratory of Radiopharmaceutical Sciences, Hokkaido College of Pharmacy
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Murakami Miho
Lab. Radiopharm. Sci. Hokkaido Coll. Pharm.
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Tanabe Tsuyoshi
Department Of Hematology/oncology The Institute Of Medical Science The University Of Tokyo
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Hayase Y
Laboratory Of Radiopharmaceutical Sciences Hokkaido College Of Pharmacy
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Takagi Yoshinari
Lab. Radiopharm. Sci. Hokkaido Coll. Pharm.
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Takahashi Tsuneo
Cell Processing Department The Institute Of Medical Science University Of Tokyo
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Kashiwakura Ikuo
Graduate School Of Health Sciences Hirosaki University
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Kashiwakura Ikuo
北海道薬科大学 放射薬品
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