Nitric Oxide Donor Sodium Nitroprusside Induces Neurotoxicity in Cerebellar Granular Cell Culture in Rats by an Independent Mechanism from L-Type or Dantrolene-Sensitive Calcium Channels(Pharmacology)

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The effects of sodium nitroprusside (SNP) in rat cerebellar granular cell culture were investigated in the present study. All doses of the SNP (10, 25, 50, 100, 250, 500μ_M) were able to induce cell death compared with control values (p<0.001 for all groups tested). Interestingly enough, a nonlinear dose-response curve was obtained for SNP-induced neurotoxicity. We also investigated the possible neuroprotective effects of nimodipine and dantrolene, alone or in combination. Both drugs failed to prevent neuronal cell death at the doses tested, either alone or in combination. Despite the fact that the most effective dose was a dantrolene concentration of 10μ_M with SNP 500μ_M and a concentration of 1μ_M with SNP 50μ_M, the differences were insignificant statistically. According to our results, SNP-induced cerebellar toxicity appears to be an independent reaction from L-type or endoplasmic reticulum calcium currents.
公益社団法人日本薬学会の論文
2002-10-01