DIFFERENT CYTOTOXICITY OF RICIN AND A CONCANAVALIN ARICIN A CHAIN CONJUGATE BETWEEN BALB/3T3 CELLS AND THE TRANSFORMED CELLS
スポンサーリンク
概要
- 論文の詳細を見る
In the previous paper, we reported that ricin and a concanavalin A-ricin A chain conjugate were more toxic to normal cell lines than the transformed variants. In this work, we confirmed this conclusion using other cell lines (KB 3 flat, ST-balb and R4-IudR) which were derived from the Balo/3T3 clone. KN7-8 had a lower number of Con A binding sites than the sensitive parent line (A-31), while the apparent association constant of Con A and surface receptors of KN7-8 was slightly higher than that of A-31. On the other hand, both cell lines had almost the same number of ricin binding sites, while the apparent association constant of KN7-8 was lower than that of A-31. These differences in the number of binding sites and the apparent association constant might not be due to the different sensitivity of these cell lines to the toxin, because the amounts of bound ^<125>I-ricin internalized into the two cell lines during incubation at 37℃ were essentially the same. Furthermore, no evidence was obtained showing that there was a difference between A-31 and KN7-8 cells in the ability of reduction of the disulfide bond of ricin, degradation of internalized toxin and in the sensitivity of the protein synthesizing machinery.
- 公益社団法人日本薬学会の論文
著者
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Beppu Masatoshi
Division Of Chemical Toxicology And Immunochemistry Faculty Of Pharmaceutical Sciences University Of
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TERAO TADAO
Division of Radiochemistry, National Institute of Hygienic Sciences
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Terao Tadao
Division Of Radiochemistry National Institute Of Hygienic Sciences
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OSAWA TOSHIAKI
Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University
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Osawa Toshiaki
Division Of Chemical Toxicology And Immunochemistry Faculty Of Pharmaceutical Sciences University Of
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Osawa Toshiaki
Laboratory Of Molecular Medicine Department Of Integrated Biosciences Graduate School Of Frontier Sc
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YAMAGUCHI TERUHITO
Central Research Laboratory, Nisshin Flour Milling Co.
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OSAWA Toshiaki
Yakult Central Institute for Microbiological Research
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Yamaguchi Teruhito
Central Research Laboratory Nisshin Flour Milling Co.
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Osawa Toshiaki
Division Of Chemical Toxicology And Immunochemistry Faculty Of Pharmaceutical Science University Of
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