Determination of SA-446 in Human Whole Blood and Urine by Electron Capture-Gas Liquid Chromatography

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A highly sensitive and specific gas chromatographic method for the determination of SA-446 [(2R, 4R)-2-(2-hydroxyphenyl)-3-(3-mercaptopropionyl)-4-thiazolidinecarboxylic acid] in human whole blood and urine was established. The mercapto group of SA-446 was alkylated completely with pentafluorobenzyl bromide (PFBB) and then the remaining hydroxyl and carboxyl groups were methylated with diazomethane. As little as 10 picograms of the derivatized SA-446 is detectable by the electron capture technique. The mean recoveries of SA-446 from whole blood and urine by the present method were 95.5% and 93.5%, respectively. The calibration curves for SA-446 were linear in the concentration ranges of 10ng/ml to 10000ng/ml of whole blood and 0.1μg/ml to 100μg/ml of urine. The standard deviation with this method was below 3.0%. This method is highly sensitive, and a small sample size of 250μl is enough to determine SA-446 in human whole blood and urine.
社団法人日本薬学会の論文
1982-06-25