Antitumor Activity of Bacillus natto. V. Isolation and Characterization of Surfactin in the Culture Medium of Bacillus natto KMD 2311
スポンサーリンク
概要
- 論文の詳細を見る
For the purpose of finding out a strain which has stronger cytolytic activity on Ehrlich ascites carcinoma cells, the authors isolated 113 strains of Bacillus natto from straws, which were collected at various areas in Japan, and measured the cytolytic activities by cylinder plate method. As the results, the authors found out a strain of Bacillus natto (tentatively called KMD 2311) which has the strongest cytolytic activity in the 113 strains. There were at least two kind of cytolytic substances on Ehrlich ascites carcinoma cells in the culture medium of Bacillus natto KMD 2311. One was extracted with AcOEt from the culture medium, which constitute approximately 20% of the cytolytic activity in the culture medium and it was stable. This cytolytic substance was purified and colorless crystalline compound (mp 247-249°) was obtained by recrystallization from acetone-petr. ether. Chemical structure of this compound was examined by elementary analysis, infrared, nuclear magnetic resonance, and mass spectroscopy and study of decomposed products. As the results, this compound was proved to be identical with surfactin (mp 140°), which was obtained from culture medium of Bacillus subtilis by Kakinuma, et al. and from Bacillus natto KMD 1126 by the authors. Melting point of this compound (mp 249°) was higher about 100° than that of surfactin (mp 140°), but high mp compound was obtained from surfactin by recrystallization from acetone-petr. ether. From these results, it was indicated that the cytolytic substance was identified with surfactin and dimorphic.
- 社団法人日本薬学会の論文
- 1974-04-25
著者
-
亀田 幸雄
Faculty of Pharmaceutical Sciences, Kanazawa University
-
松井 勝彦
Faculty of Pharmaceutical Sciences, Kanazawa University
-
金友 昭一
北陸大学薬学部
-
金友 昭一
School Of Pharmacy Hokuriku University
-
金友 昭一
Faculty Of Pharmacy Kanazawa University
-
亀田 幸雄
School Of Pharmacy Hokuriku University
-
松井 勝彦
Faculty Of Pharmacy Hokuriku University
-
大平 貞夫
Faculty of Pharmaceutical Sciences, Kanazawa University
-
長谷 哲
Faculty of Pharmaceutical Sciences, Kanazawa University
-
阿津坂 巧
Faculty of Pharmaceutical Sciences, Kanazawa University
-
長谷 哲
Faculty Of Pharmaceutical Sciences Kanazawa University
-
大平 貞夫
Faculty Of Pharmaceutical Sciences Kanazawa University
-
阿津坂 巧
Faculty Of Pharmaceutical Sciences Kanazawa University
関連論文
- Synthesis of Actinomycin Related Compounds. I
- Sparsomycin Analogs. III. Synthesis and Biological Activities of (E)-β-(6-Methyluracil-5-yl) acrylic Acid Derivatives
- Sparsomycin Analogs. II. Synthesis and Biological Activities of 5-Carboxy-6-methyluracil Derivatives
- 環状デプシペプチドSurfactinに関する研究(第1報)納豆菌(Bacillus natto KMD 2311)の産生する8種Surfactin同族体の単離と構造
- Sparsomycin Analogs.VI. : Synthesis and Antitumor Activity of Octylsparsomycin Analogs
- 納豆菌の抗腫瘍性(第6報)納豆菌の産生するエールリッヒ腹水癌細胞溶解活性の解析
- Enzymatic Synthesis of Glucoside Derivatives of Validamine and Valienamine
- Inhibitory Effect of Validamine, Valienamine and Valiolamine on Activities of Carbohydrases in Rat Small Intestinal Brush Border Membranes
- Chemical Modification by 2,4,6-Trinitrobenzenesulfonic Acid (TNBS) of an Essential Amino Group in 3-Ketovalidoxylamine A C-N Lyase
- Purification and Some Properties of Cytochrome c_ from Flavobacterium saccharophilum
- Fluorometric Studies on the Role of Calcium in Substrate Binding to 3-Ketovalidoxylamine A C-N Lyase
- Chemical Modification of Amino Groups in Alanine Dehydrogenase from Bacillus natto KMD 1126
- Reactivity and Function of Sulfhydryl Groups in Alanine Dehydrogenase of Bacillus natto KMD 1126
- Purification and Properties of Alanine Dehydrogenase from Bacillus natto KMD 1126
- Purification and Properties of Leucine Dehydrogenase of Bacillus natto KMD 1126
- Antitumor Activity of Bacillus natto. V. Isolation and Characterization of Surfactin in the Culture Medium of Bacillus natto KMD 2311
- Antitumor Activity of Bacillus natto. IV. Purification and Properties of an Extracellular Protease from Bacillus natto KMD 1126
- Antitumor Activity of Bacillus natto. III. Isolation and Characterization of a Cytolytic Substance on Ehrlich Ascites Carcinoma Cells in the Culture Medium of Bacillus natto KMD 1126
- Antitumor Activity of Bacillus natto. II. Formation of Cytolytic Substances on Ehrlich Ascites Carcinoma in Bacillus natto KMD 1126
- Studies on Acylase Activity and Micro-organisms. XXV. Purification and Properties of Benzoylamino-acid Amidohydrolase in KT 801 (Pseudomonas sp.)
- Studies on Acylase Activity and Microorganisms. XXIV. Properties of δ-Ornithine Acylase : 5-N-Acylornithine Amidohydrolase.
- Studies on Acylase Activity and Microorganisms. XXIII. Purification of δ-Ornithine Acylase : 5-N-Acylornithine Amidohydrolase.
- Studies on Acylase Activity and Microorganisms. XXII. Isolation of Soil Bacteria Capable of Producing δ-Ornithine Acylase : 5-N-Acylornithine Amidohydrolase.
- Studies on Acylase Activity and Microorganisms. XIX. Optical Resolution of Threonine, Lysine, Tryptophan, and Methionine by Acylase of Soil Bacteria.
- Purification and Properties of Acylase from Ehrlich Ascites Carcinoma Cells
- Studies on Acylase Activity and Micro-organisms. XXVII. Purification and Properties of Phenylacetyl DL-Acylase (N-Phenylacetyl-DL-amino-acid Amidohydrolase) from AAA 6020 (Pseudomonas sp.)
- Studies on Acylase Activity and Micro-organisms. XXVI. Purification and Properties of D-Acylase (N-Acyl-D-amino-acid Amidohydrolase) from AAA 6029 (Pseudomonas sp.)
- Optical Resolution of Methionine and ε-N-Benzoyl-lysine by Metabolism of Escherichia coli on Phenylacetyl Derivatives
- Hydrolysis and Metabolism by Soil Bacteria of Acetyl-D-leucinamide
- A Contact Antitumor Activity of Bacillus natto on Solid Type Ehrlich Carcinoma Cells
- Studies on Acylase Activity and Microorganisms. XX. Isolation of Soil Bacteria Capable of Resolving Tryptophan by their Acylase on N-Acetyl-DL-tryptophan.
- Sparsomycin Analogs. IV. : Synthesis and Antitumor Activity of Pyrimidine-5-carboxamides and (E)-β-(Pyrimidin-5-yl)-acrylamides
- Sparsomycin Analogs. I. Synthesis of 5-Carboxy-6-methyluracil
- Studies on 1-Azabicyclo Compounds. XXVIII. Synthesis of 1-Methyl-perhydroazocin-5-one from Pyrrolizidine
- The Polyphosphoric Acid-Catalyzed Ring Opening of 4,5-Epoxy-3-oxo Steroids : The Synthesis of 4-Alkylthio-4-en-3-oxo Steroids and their Analogs
- Studies on Acylase Activity and Microorganisms. XVII. Optical Resolution of Tryptophan, 2-Aminohexanedioic Acid, and 2-Aminooctanoic Acid by Metabolism of Soil Bacteria on Benzoyl Derivatives of DL-Amino Acids.
- Studies on Acylase Activity and Microorganisms. VIII. Enzymatic Hydrolysis of 6-N-Benzoyl-L-lysine.
- Alkaline Decomposition of Enmein.
- エンメインの構造に関する化学的研究
- 日本薬学会教育賞受賞越浦良三氏の業績
- Sparsomycin Analogs. V. : Synthesis and Antitumor Activity of (E)-β-(Pyrimidin-5-yl)acrylamides
- ortho-Directed Lithiation of (2-Methoxy) ethoxy- and (2-Dimethylamino) ethoxy-arenes
- Studies on Acylase Activity and Microorganisms. XI. A New Method for resolving DL-Amino Acids by Metabolism of Soil Bacteria.
- Studies on Acylase Activity and Microorganisms. X. Enzymatic Resolution of Alloisoleucine by Soil Bacteria, KT 85 (Pseudomonas sp.).
- Studies on acylase activity and microorganisms.-22〜24-