Chemical Modification by 2,4,6-Trinitrobenzenesulfonic Acid (TNBS) of an Essential Amino Group in 3-Ketovalidoxylamine A C-N Lyase
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概要
- 論文の詳細を見る
3-Ketovalidoxylamine A C-N lyase of Flavobacterium saccharophilum is a monomeric protein with a molecular weight of 36000,and contains 32 amino groups and no cysteine or cystine residues. The enzyme was inactivated by 2,4,6-trinitrobenzenesulfonic acid (TNBS) following pseudo-first order kinetics. Substrate of the lyase, p-nitrophenyl-3-ketovalidamine, protected the enzyme against the inactivation, suggesting that the modification occurred at or near the active site. Although several amino groups were modified by TNBS, a plot of log (reciprocal of the half-time of inactivation) versus log (concentration of TNBS) suggested that one amino group has an essential role in catalysis.
- 社団法人日本薬学会の論文
- 1990-05-25
著者
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松井 勝彦
Faculty Of Pharmacy Hokuriku University
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松井 勝彦
Department of Microbiology, Meiji College of Pharmacy
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竹内 正義
Department of Biochemistry, School of Pharmacy, Hokuriku University
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閨崎 憲一
Department of Biochemistry, School of Pharmacy, Hokuriku University
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竹内 正義
Department Of Biochemistry School Of Pharmacy Hokuriku University
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松井 勝彦
Department Of Biochemistry School Of Pharmacy Hokuriku University
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閨崎 憲一
Department Of Biochemistry School Of Pharmacy Hokuriku University
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