Antioxidant Effect of Vitamin K Homologues on Ascorbic Acid/Fe^<2+>-induced Lipid Peroxidation of Lecithin Liposomes
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概要
- 論文の詳細を見る
The effects of vitamin K homologues (K_1,K_2 and K_3) on lipid peroxidation of lecithin liposomes induced by ascorbic acid and ferrous ion were examined. Ubiquinone-10 (IQ-10) was used as a reference in evaluation of the effectiveness of these vitamins. The lipid peroxidation was assessed by measurements of thiobarbituric acid-reactive substance (TBARS) and conjugated diene formation during the reaction. Among them, vitamins K_1 and K_2 inhibited the lipid peroxidation, as did UQ-10,with the order of effectiveness : UQ-10>K_2>K_1. By contrast, vitamin K_3 had no inhibitory effect on ascorbic acid/Fe^<2+>-induced lipid peroxidation of the liposomes. The inhibitory effect of vitamins K_1 and K_2 appeared only when these vitamins were incorporated into the liposomes by sonication. Simple mixing of the liposomes with these vitamins or with UQ-10 did not inhibit peroxidation of the liposomes even at high concentrations. From measurements of nitroblue tetrazolium reduction and p-nitrosodimethylaniline bleaching of vitamin K_1- or K_2-incorporated liposomes in the presence of ascorbic acid/Fe^<2+>, it was found that these vitamins prevent the formation of hydroxyl radicals, not superoxide anions, during the peroxidation reaction. However, the degree of ascorbic acid/Fe^<2+>-induced TBARS formation of the liposomes was not inhibited by the addition of mannitol to the reaction mixture. From these results, it is suggested that the inhibitory effect of these vitamins is mainly involved in termination of radical-chain reaction. Experimantal results using several radical scavengers and/or antioxidants supported this interpretation. The treatment of vitamin K_2-incorporated liposomes with ascorbic acid/Fe^<2+> resulted in a decrease of the absorbance at 328 nm of the vitamin, depending on the incubation time. In addition, it was found that there is a linear relationship between the degree of the absorbance change at 328 nm and the amount of TBARS formed at different incubation times of the liposomes with ascorbic acid/Fe^<2+>.
- 社団法人日本薬学会の論文
- 1991-04-25
著者
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松井 勝彦
北陸大・薬
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松井 勝彦
Department of Microbiology, Meiji College of Pharmacy
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大屋敷 孝雄
北陸大・薬
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大屋敷 孝雄
Department of Biochemistry, School of Pharmacy, Hokuriku University
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藪中 祥寿
Department of Biochemistry, School of Pharmacy, Hokuriku University
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藪中 祥寿
Department Of Biochemistry School Of Pharmacy Hokuriku University
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松井 勝彦
Department Of Biochemistry School Of Pharmacy Hokuriku University
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