Purification and Some Properties of Cytochrome c_<550> from Flavobacterium saccharophilum

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Cytochrome c_<550> was purified to electrophoretic homogeneity by ion-exchange chromatography and gel filtration from a soluble fraction of Flavobacterium saccharophilum. The purified cytochrome c_<550> had a molecular weight of 12500 as determined by gel filtration and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Cytochrome c_<550> possessed absorption peaks at 407 nm in the oxidized form, and at 415,521,550nm in the reduced form, Soluble D-glucoside 3-dehydrogenase reduced the purified cytochrome c_<550> with sugar, and this sugar was converted to the corresponding 3-keto sugar. The purified cytochrome c_<550> was autoxidizable. This shows that cytochrome c_<550> may be an in vivo electron acceptor for soluble D-glucoside 3-dehydrogenase.
社団法人日本薬学会の論文
1990-04-25