Studies on Acylase Activity and Micro-organisms. XXVI. Purification and Properties of D-Acylase (N-Acyl-D-amino-acid Amidohydrolase) from AAA 6029 (Pseudomonas sp.)

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AAA 6029 (Pseudomonas sp.) was isolated from soil by using the synthetic medium containing N-benzoyl-D-phenylalanine as a sole source of carbon. The bacteria produce a D-acylase which hydrolyzes N-acyl-D-amino acids. The D-acylase was extracted by means of sonic oscillation and purified by ammonium sulfate fractionation, DEAE cellulose chromatography, and Sephadex G-100 gelfiltration. The purified enzyme was represented about 900 fold purification over the cell free extract. The molecular weight of this enzyme was estimated to be about 45000 by gelfiltration. This enzyme can hydrolyze N-benzoyl and N-acetyl derivatives of the D-form of phenylalanine, methionine, leucine, alanine, and valine. But the acylase can not hydrolyze N-acyl derivatives of L-amino acids.
社団法人日本薬学会の論文
1978-09-25