Image Analysis of Separation Processes. I. Development of a Video Image Analyzing System and Its Fundamental Application to the Direct Observation of a Separation Process in a Column

概要

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In order to analyze the separation processes taking place in liquid chromatography, a video image analyzing system was developed. A video camera, video tape recorder and an image processor were linked to a microcomputer. The distribution of fluorescent solutes in a glass column was measured as a densitogram which was regarded as a chromatogram inside the column. The apparent migration rate, which was the migration distance for a unit migration volume, was determined by this method and was found to have a good linear relationship with the capacity factor k'. Band broadening during separation was also estimated at various flow rates. It was concluded that the band broadening was mainly due to multiple-path diffusion.
社団法人日本薬学会の論文
1994-03-15