MOBILE PHASE EXCHANGE PRECESS IN STEPWISE ELUTION LIQUID CHROMATOGRAPHY ANALYZED BY CHROMATO-VIDEOSCOPE

概要

論文の詳細を見る
Dynamic aspects of the solvent exchange process in stepwise elution liquid chromatography were investigated using a chromato-videoscope system. The migration velocity of a solute's band changed rapidly at the same flow rate when a second eluent overtook the band. The changing time of the migration velocity is termed an inflective time. Since a plot of the relationship between inflective time and the migration distance up to the overtaking point of each solute band showed a straight line, the solvent front of the second eluent seemed to be sharp during passage through the column. In the case where the content of organic solvent in the mobile phase was changed from higher to lower, there was a delay of the inflective time even though the solvent front of the second eluent seemed to be kept sharp.
社団法人日本薬学会の論文
1994-11-15