Effects of Choline and Inositol on ^3H-Dihydroalprenolol and ^<125>I-Iodocyanopindolol Bindings to β-Adrenegic Receptors of the Guinea Pig Cerebral Cortical Membranes
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概要
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This experiment was designed to examine the effects of choline and inositol in Eagle's minimal essential medium (MEM), because the values of specific binding were increased when the ^3H-dihydroalprenolol (^3H-DHA) binding assay of β-adrenoceptors in the guinea pig and rat cerebral cortical membranes was carried out in Eagle's MEM instead of 60 mM Tris-HCl, 20 mM MgCl_2 buffer (pH 7.4). All chemicals contained in the MEM were added to the ^3H-DHA binding mixture and the values of the specific binding were compared with the control values obtained in the absence of the chemicals. The presence of choline (0.1-10 μM) or inositol (0.1-10 μM) induced an increase of the values of the specific binding. These effects of choline and inositol were observed after preinclubation for 20 and 1 min, respectively. After 30 min of incubation, no changes were observed upon removal of these chemicals. Furthermore, choline and inositol significantly increased the β-adrenoceptor density (β<max>) when ^3H-DHA and <125>I-iodocyanopindolol were used as the radioligands, as determined by Scatchard analysis, but no significant change was observed in the value of the dissociation constant (K_d) in ^3H-DHA binding. These results suggest that choline and inositol in the membranes could have a crucial role in drug-receptor interaction.
- 社団法人日本薬学会の論文
- 1988-01-25
著者
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長友 孝文
Department of Pharmacology, Niigata College of Pharmacy
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土橋 洋史
Department of Pharmacology, Niigata College of Pharmacy
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長友 孝文
新潟薬科大学薬学部薬理学教室
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土橋 洋史
ひまわり薬局
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土橋 洋史
新潟薬科大学 薬理学教室
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長友 孝文
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App
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