Estimation of the Molecular Weight of Ions by Isotachophoresis. II. : Influence of Electric Charge on the Precision of the Correlative Equations

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Correlative equations relating the molecular weight of ions of some organic acids and amino acids to the relative step height (or R_E) in isotachopherograms were derived. The equation for organic acids was R_E=0.580+0.016M/|Z|, and that for amino acids was h_R=0.500+0.021 M/|Z|, where the relative step height (h_R) is the ratio of the step height of the sample ion to that of chloride ion, R_E is the ratio of the potential gradient of the sample zone to that of the leading zone, and M and Z are the molecular weight and the electric charge of ions, respectively.A good inverse proportionality of electric charge (Z) to R_E or h_R was confirmed, even though dissociation was not complete. The slope value (b) of the correlative equation for each ion may represent the ratio of the resistance of the ion the molecular weight, R_E/M or h_R/M, and may depend on the structure of the ion.The h_R-pH curves based on the equation for amino acids and the experimental results showed that the order of h_R value was the same as that of molecular weight for Gly, Gly_2,and Gly_3 in the case of pH_L 10.2.
社団法人日本薬学会の論文
1986-03-25