Preparation of Specific Antisera to 3β-Hydroxy-5-cholenoic Acid by Immunization with Conjugates Attached to Protein through the C-3 Position

概要

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3β-Hydroxy-5-cholenoic acid-bovine serum albumin (BSA) conjugates were prepared by coupling of the haptens with the carrier protein through a 3-hemisuccinoyl or 3-hemiglutaroyl bridge. The antisera elicited by these new immunogens showed high affinity constants (K_a=4.88×10^8 and 8.75×10^8 M^<-1>) and excellent specificity to 3β-hydroxy-5-cholenoic acid, exhibiting little or no cross-reactivities with cholesterol (0.001%) and related compounds. Though the antiserum obtained with the hemisuccinate-BSA conjugate was somewhat more specific than the other, they showed substantially the same reactivity, and should be useful for direct radioimmunoassay of 3β-hydroxy-5-cholenoic acid in biological fluids without any chromatographic separation step prior to assay.
公益社団法人日本薬学会の論文
1985-02-25