Electron Spin Resonance Detection of Singlet Oxygen Produced in Irradiated Acriflavine Solutions

概要

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When a solution of acriflavine was irradiated, singlet oxygen was detected by electron spin resonance (ESR). When a 2,2,6,6-tetramethyl-4-piperidone solution (TEMP) was irradiated in the presence of acriflavine (AF) or hematoporphyrin (HP), a signal was detected and identified as being due to produced 2,2,6,6-tetramethyl-4-piperidone-N-oxyl (TEMPO) from a comparison of the g values and the hyperfine splitting constants with the nitrogen nuclear spin with those of an authentic sample. HP produced TEMPO more efficiently than AF at the same concentration (10^<-4>м). Prolonged irradiation brough about rapid destruction of TEMPO as indicated by the disappearance of the TEMPO signal. This phenomenon may be due to the quenching of nitroxide radical by the decomposition product of TEMP. However, TEMPO itself was very stable in the presence or absence of AF even under such drastic conditions as irradiating the sample with ultraviolet (UV) light at a distance of 0.5 cm for 60 min. Maximum TEMPO production was obtained when the TEMP solution was irradiated by the UV lamp for 25 min from 3 cm distance in the presence of 10^<-5>м AF.
社団法人日本薬学会の論文
1985-12-25