Isolation of Antimycin-resistant Variants of Candida utilis

概要

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In the process of isolating several antimycin-resistant mutants of Candida utilis by treatment with N-methyl-N'-nitro-N-nitrosoguanidine, we found a simple method of isolating many antimycin-resistant variants without treatment with mutagen. When cells were grown in glucose medium containing 10^<-6> M antimycin for more than three days at 30°, we observed the existence of some antimycin-resistant variants in the culture medium. These variants can grow in glycerol medium containing 10^<-6> M antimycin in which the parental strain can not grow. The results of fluctuation test strongly suggested that antimycin-resistant variants arose unperiodically by spontaneous mutation. The respiration of antimycin-resistant variants was not inhibited by 10^<-7> M antimycin which inhibited completely that of parental strain, but was inhibited by the higher concentrations of antimycin. Therefore, these variants do not seem to become antimycin resistant by the possession of any special antimycin-resistant respiratory chain as reported on several fungi. Some other possibilities were discussed.
社団法人日本薬学会の論文
1978-10-25