Radioimmunoassay for N^α-(N-Acetylmuramyl-alanyl-D-isoglutaminyl)-N^ε-stearyl-lysine

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A radioimmunoassay for N^α-(N-acetylmuramyl-alanyl-D-isoglutaminyl)-N^ε-stearyl-lysine (MDP-Lys-L18) was established. Antibody to MDP-Lys-L18 was obtained from rabbits by immunization with MDP-Lys-L18 conjugated with bovine serum albumin (BSA). ^3H-MDP-Lys-L18 labelled at C_9 and C_<10> of the stearyl residue was used. The ammonium sulfate method was adopted for the separation of bound and free fractions. Though the protein binding of MDP-Lys-L18,which was due to the stearyl residue in the molecule, influenced the sensitivity of the radioimmunoassay, the lower limit for the quantitative determination of MDP-Lys-L18 in serum was estimated to be 500pg/ml and the coefficient of variation of this assay was between 3 and 5%.
1984-10-25