Inhibition by 3-Amino-1,2,4-triazole of Fatty Acid Synthesis in Cell Free System

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In this paper, the mechanism of inhibition by aminotriazole (3-amino-1,2,4-triazole) of fatty acid synthesis was studied with cell free system of the ratliver. The concentration of this compound in the liver was 20 mM at 30 min after the injection of the compound (100 mg/100 g, i.p.). About 80% of this compound in the liver cell was recovered in the supernatant fraction (cytoplasm) by subcellular fractionation of the liver homogenate. In the experiment of cell free system, the incorporations of ^<14>C-acetate and ^<14>C-acetyl-CoA into fatty acid were inhibited about 50% by the addition of 20 mM aminotriazole into the system and this inhibition depended on the concentration of the compound. However, the incorporation of ^<14>C-malonyl-CoA into fatty acid was not inhibited by aminotriazole. Aminotriazole repressed the activation and stabilization of acetyl-CoA carboxylase in the rat liver by citrate and inhibited this enzyme non-competitively.
1978-09-25