Syntheses of Halogen Substituted Indolyl β-D-Glucuronides and Their Hydrolysis by Rabbit Liver β-Glucuronidase
スポンサーリンク
概要
- 論文の詳細を見る
I and II were synthesized as their barium salts. Methyl (1-bromo-tri-O-acetyl-α-D-glucoside) uronate was condensed with haloindolyl acetate in dry methanol containing sodium methoxide. Acetylation of crude methyl (haloindol-3-yl-β-D-glucoside) uronate thus obtained, followed by deacetylation and demethylation with aq. alkali acetone, afford the desired haloindol-3-yl-β-D-glucuronides. The hydrolysis of I and II by rabbit liver β-glucuronidase was investigated. The rate of hydrolysis depends on the nature of the buffer, and decreases in the following order : acetate>citrate-phosphate>phosphate. The optimum pH was 4.75 in acetate buffer and 5.0 in both citrate-phosphate and phosphate buffer. K_m for I and II is 8×10^<-4>M and 5×10^<-3> M, respectively. I was applied to the demonstration of β-glucuronidase in disk electrophoresis.
- 社団法人日本薬学会の論文
- 1975-08-25
著者
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吉田 和夫
Daiichi College Of Pharmaceutical Sciences
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飯野 信子
Daiichi College of Pharmaceutical Sciences
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古賀 生榮
Daiichi College of Pharmaceutical Sciences
関連論文
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