Synthesis of β-D-Galactofuranosides and Their Behavior toward β-Galactosidase

概要

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After ethanolysis of galactose with Dowex-50 as catalyst, ethyl β-D-galactofuranoside, ethyl β-D-galactopyranoside and ethyl α-D-galactopyranoside were isolated by cellulose column chromatography. m-Tolyl and guaiacol β-D-galactofuranoside were also prepared. Ethyl, phenyl, m-tolyl and guaiacol β-D-galactofuranoside were hydrolyzed by almond emulsin β-galactosidase, but not hydrolyzed by E. coli K 12 and bovine liver β-galactosidase. The kinetics of the hydrolysis of β-D-galactofuranoside by almond emulsin β-galactosidase were investigated. The pH optimum of the β-D-galactofuranoside is pH 5.0 in phosphate-citrate buffer. The Michaelis constant of phenyl β-D-galactofuranoside is 5.6×10^<-2>M. The kinetics of the hydrolysis of corresponding β-D-galactopyranoside were also investigated. The pH optimum is pH 4.5-5.0 for almond emulsin β-galactosidase, pH 5.5 for bovine liver β-galactosidase and pH 7.0-7.5 for E. coli K 12 β-galactosidase. The Michaelis constant of phenyl β-D-galactopyranoside for almond emulsin β-galactosidase is 3.2×10^<-3>M. The Michaelis constant of phenyl β-D-galactopyranoside is smaller than that of corresponding furanoside.
社団法人日本薬学会の論文
1969-06-25