Opalinaの局部化学刺激による繊毛打敲方向変化の機構の解析 : I.Kイオンの作用機構

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Obser vation of the ciliary reactions and measurement of the electric current through the cell membrane were made during the local application of KCl solution to the cell body of Opalina. The results obtained were summarized as follows. 1. At the moment of Local application of 15 mM KCl solution to Opalina adapted in Ringer's solution, the cilia on the applied area continued to beat in normal direction. On the other hand, beating direction of the cilia on the rest of the cell surface was reversed. 2. The degree of ciliary reversal induced by localapplication of KCl solution differed from part to part to which the KCl solution was applied. KCl locally applied to the left side of the anterior part of the animal always induced a violent ciliary reversal, while that to the right side induced only a weak reversal. 3. There was a topographical difference in the degree of ciliary reversal induced by local application of KCl at a fixed part on the cell surface. Such a difference seemed to be in accord with the topographical difference of electrical sensitivity of the cell surface. 4. Local application of 15 mM KCl solution to Opalina adapted in 99 mM KCl solution induced the augmentation of normal stroke of cilia on the general surface of the cell, except the area to which 15mM KCl was applied. At the moment of cessation of the stimulation, the cilia reversed their beating direction temporally. The beation of cilia onthe 15 mM KCl applied area were scacely changed during and after the application. 5. The measurements of the electric current through the membrane during the local application of 15mM KCl solution demonstrated that the membrane current flewoutward and was strong enough to induce the ciliary reversal when the organism was in Ringer's solution, while it flew inward and was strong enough to induce the augmentation of the normal stroke of cilia when the organism was in 99 mM KClsolution. 6. From these results, it was concluded that the ciliary response evoked by the local application of KCl solution was due to the membrane current generated by the discharge of the membrane potential through the area depolarized by KCl solution. 7. Ciliary reversal induced by the external application of KCl solution to whole animal seemed to be attributable to the topographical difference in the extent of the depolarization by KCl solution. 8. The possibility that the so-called reversal impulses in ciliate protozons was to be taken as the discharge of membrane potential was discussed. 9. The fact that the tip of the cell was far more sensitive to KCl stimulation than the rest of the cell was regarded to be favourable for the establishment of avoiding reaction in Opalina.
社団法人日本動物学会の論文
1961-12-15

Opalinaの局部化学刺激による繊毛打敲方向変化の機構の解析 : I.Kイオンの作用機構

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