外液に加えた栄養物質のOpalinaの生存時間に対する影響

概要

論文の詳細を見る
1) In order to find out an adequate culture medium for Opalina, collected from Rana japonica, effects on survival time of the animal were determined by the addition of a few kinds of nutrients to the physiological saline solution in which Opalina were suspended. 2) Maximum concentrations of penicillin-Na G and of dihydrostreptomycin sulfate which did not affect the survival time of Opalina were 4,000u/cc and 10mg/cc respectively. 3) In the saline containing 0.2mM glucose, the survival time was almost twice as long as that in the saline containing no glucose. 4) Addition of 8mM sodium phosphate to the glucose saline solution increased the survival time up o 200%. 5) Further addition of hydrolyzed casein (1,000γN/cc) to the solution containing glucose and phosphate markedly increased the survival time up to more than 1,000%. However, active multiplication of the cell could not be observed. 6) Addition of slight amounts of yeast extract to the solution showed no marked effects on the survival time.
社団法人日本動物学会の論文
1964-10-15