Opalinaの生理的塩溶液の決定

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1. In order to determine a physiological saline solution for Opalina, collected from the rectum of Rana japonica, the effects of various factors (osmotic pressure, viscosity, pH, cations and anions) of external solution on the survival time of the animal were examined. 2. Time required for 50% of the treated animals to cease their ciliary motion was taken to represent the survival time. 3. 50-100 animals with 0.5cc test solution were introduced between a hollow slide and a cover glass sealed with vaseline. 4. The most adequate tonicity for Opalina was found to be 75mM NaCl equiv. The toniciy range from 40 to 100mM NaCl equiv. gave the survival time longer than 80% of the longest one in 75mM NaCl solution. 5. The most favourable pH value for Opalina was 7.8. The pH range from 6.2 to 8.8 gave the survival time longer than 80% of the longest one in the soultion of pH 7.8. 6. The survival time was no offected by [K]/[Na] ratio of the external solution. 7. External Ca-concentration lower than 10^<-4>M was found to be enough to maintain normal survival time. Ca of high concentration (above 10^<-2>M) rather decreased the survival time. The presence of 10^<-3>M MgCl_2 in the external solution clearly increased the survival time. 8. SO_3 and thioglycollate (60mM) markedly increased the survival time. In the solution containing 60mM NaF, survival was almost perfectly depressed. On the other hand, the depression effect of NaCN (60mM) was far less than that of NaF. These results seemed to suggest the anaerobic nature of life processes of Opalina. 9. An increase in external viscosity up to 200 times that of pure water scarcely affected the survival time, however, a slight increase in survival time was observed in a more viscous solution (relative viscosity, 600). 10. The author wishes to propose a solution with the following composition as one of the most adequate physiological salines for Opalina; NaCl, 60mM; KCl, 5mM; CaCl_2, 0.1mM; MgCl_2, 1mM; tris buffer, 10mM (pH 7.8).
社団法人日本動物学会の論文
1964-09-15

Opalinaの生理的塩溶液の決定

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