ヒトデの放卵を誘起する神経物質の安定性について

概要

論文の詳細を見る
With respect to the thermo-stability of the gamete-shedding substance obtained from radial nerves of starfish, some contradictory results have been reported. Kanatani and Noumura (1962) have shown that the active substance is considerably heat-stable. Chaet and Rose (1961) also reported that the substance was only partially inactivated by boiling for 20 minutes. On the contrary, Chaet (1966 a, b, 1967) has recently reported that the shedding substance is rather heat-labile, that is, water extracts lose their activity within 18 hours at room temperature or 7 days at 5 or 7℃. To test the possibility that this result was due to bacterial contamination, the stability of the shedding substance in relation to heating and asepsis was studied in Asterina pectinifera, using isolated ovarian fragments as assay material. The gamete-shedding activity of water extracts of lyophilized nerve was found to remain intact for at least 23 days at room temperature (about 23℃) when preheated (100℃ for 10 min or 80℃ for 30 min) and kept in an aseptic condition. On the other hand, control samples without such pre-treatments lost their activity within 24 hours ats 23℃. Use of nbutanol as a stabilizer of the shedding substance was found to be successful; aqueous solutions of the shedding substance containing 1% or 2% butanol retained their activity for at least 9 days at 23℃.
社団法人日本動物学会の論文
1968-04-15