カルシウム欠除海水中におけるイトマキヒトデ卵の1-メチルアデニンによる成熟

概要

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In starfish, a gonad-stimulating substance (GSS) contained in the nervous tissue acts on the ovary to produce a meiosis-inducing substance (MIS) which induces oocyte maturation. The desalted supernatant of the incubation mixture of the ovarian fragments and sea water containing GSS induces maturation in isolated oocytes of Asterina pectinifera even in calcium-free sea water (Kanatani, 1967). On the other hand, Schuetz (1969) reported that washing ovarian fragments of Asterias forbesi in calcium-free sea water partially inhibited the action of MIS. The present study was carried out in order to determine whether lack of calcium ions in sea water has any effect in inducing oocyte maturation in the presence of various concentrations of 1-methyladenine (1-MA), which has been shown to be the MIS of Asterias amurensis (Kanatani et al., 1969). The data (Table 1 and 2) show that at least in Asterina pectinifera, lack of calcium ions had no effect on the meiosis-inducing activity of 1-MA. However, when the ovarian fragments of Asterias amurensis were used as material, oocyte maturation within the ligated fragments in calcium-free sea water required larger doses of 1-MA than in the case of normal sea water. A sufficient amount of 1-MA, however, induced 100% oocyte maturation even in the calcium-free sea water (Table 4).
社団法人日本動物学会の論文
1970-05-15