The Role of NK Cells in the Elicitation Phase of Oxazolone Inducing Contact Hypersensitivity

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概要

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• We studied the role of infiltrating cells and cytokines synthesis in oxazolone (OXA)-induced contact hypersensitivity (CMS) of C57BL/6 mouse ear model. The cell mediate lesion was induced by the OXA challenge after abdominal skin sensitization, and those of infiltrating cells were examined at various time points (0-168 hr). The CMS lesions were indicated as an ear swelling at 24 to 48 hr after the challenge that was caused mainly by infiltration of mononuclear cells expressing CD90^+, CD4^+ and CD8^+. However, NK cells by means of asialo GM1^+ cells, were detected in the lesions as early as 2 hr after challenge. The infiltration of NK cells peaked at 8 hr and replaced in the predominant accumulation of polymorphonuclear (PMN) cells and some of CD90^+ cells. Cytokine expression of the infiltrating cells by RT-PCR, revealed a high level of IL-2 expression at 8, 12, and 24 hr and then reduced expression in the following times, while, IFN-_γ was constantly detected from 2 hr to 168 hr. Deletion of the NK cell activity by administration of the anti-asialo GM1 antibody resulted in significantly suppressed cell infiltration and ear swelling. IL-2 mRNA expression was undetected in the infiltrating cells of deletion mouse, whereas IFN-_γ was detected at 12, 24, and 48 hr. The presence of NK cells as the first infiltrating cells in the lesion and IL-2 and INF-_γ synthesis from the infiltrating cells may play an important role in the elicitation phase of CHS.

• 日本組織細胞化学会の論文

著者

• MORO Itaru

  Department of Pathology, Nihon University School of Dentistry

• Moro I

  Department Of Pathology Nihon University School Of Dentistry

• Moro Itaru

  Department Of Pathology Nihon University School Of Denistry

• KOMIYAMA Kazuo

  Department of Pathology, Nihon University School of Dentistry

• Komiyama Kazuo

  日本大学 歯学部口腔外科学第2

• Komiyama Kazuo

  Department Of Oral And Maxillofacial Surgery Nihon University School Of Dentistry

• KANEKO Takahiro

  Department of Bioengineering, Akita Research Institute of Food and Brewing (ARIF)

• Okaue Masahiro

  Department of Pathology and Oral Surgery, Nihon University School of Dentistry

• Kaneko T

  Department Of Oral And Maxillofacial Surgery Saitama Medical Center Saitama Medical School

• Okaue Masahiro

  Department Of Oral And Maxillofacial Surgery Nihon University School Of Dentistry

• Kaneko Tomoatsu

  Pulp Biology And Endodontics Dept. Of Restorative Sciences Graduate School Tokyo Medical And Dental

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