Streptococcus mutans g 型菌に dextranase は存在するか

概要

論文の詳細を見る
An extracellular dextranase has been detected and isolated from Streptococcus mutans AHT (serotype g). Dextranase was partially purified from the culture supernatant of S. mutans AHT by precipitation with ammonium sulfate, chromatography on DEAE-cellulose. DEAE-cellulose column chromatography permitted the removal of a large proportion of the extracellular glucosyltransferases. But glucosyltransferase isozyme (pI 4.2) remained with the dextranase solution. Dextranase activity in DEAE-cellulose fraction was detected by method of agar plate containing DT2000 and newly method of polyacrylamide gel electrophoresis containing Blue dextran. The remaining glucosyltransferase isozyme (pI 4.2) in DEAE-cellulose fraction was attempted to separate from dextranase by filtration on Ultrogel AcA34, fast protein liquid chromatography (FPLC, Mono Q column). But we could not completely separate glucosyltransferase isozyme (pI 4.2) from dextranase.
九州歯科学会の論文
1986-02-25