Mechanisms Underlying Nano-Sized Air-Pollution-Mediated Progression of Atherosclerosis : Carbon Black Causes Cytotoxic Injury/Inflammation and Inhibits Cell Growth in Vascular Endothelial Cells
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概要
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Background Epidemiological studies indicate a significant link between exposure to environmental air pollution and mortality and morbidity from ischemic heart disease. Because nanoparticles can translocate into blood circulation, the present study aimed to clarify their direct effects on human vascular endothelial cells (ECs). Methods and Results Human umbilical vein ECs (HUVECs) were treated with carbon black (CB), a component of diesel exhaust particles, for 24 h. CB induced cytotoxic morphological changes such as cytosolic vacuole formation, cell disorientation and decreased density. Lactate dehydrogenase assay revealed that CB induced cytotoxic injury in both the cells and plasma membranes. Proliferation assay showed that CB inhibited cell growth. Monocyte chemoattractant protein-1 but not vascular cell adhesion molecule-1 was induced by CB. CB reduced the expressions of connexin37 and endothelial nitric oxide (NO) synthase. Microarray analysis revealed the induction of pro-inflammatory molecules by CB. Conclusions The present results demonstrate for the first time that CB directly affects the endothelium, causing cytotoxic injury, inflammatory responses, and inhibition of cell growth. As EC injury/inflammation and membrane disintegration are related to the initiation of atherosclerosis, and NO is anti-atherogenic and anti-thrombogenic, the direct effects of nanoparticles on ECs may represent one mechanism behind environmental air pollution-mediated atherosclerosis and ischemic heart disease.
- 社団法人日本循環器学会の論文
- 2005-12-20
著者
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IWAI Naoharu
Department of Epidemiology, Research Institute, National Cardiovascular Center
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Iwai Naoharu
Department Of Epidemiology National Cardiovascular Center
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Yamawaki Hideyuki
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences The Universit
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Yamawaki Hideyuki
Department Of Epidemiology Research Institute National Cardiovascular Center
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