キュウリの微少組織を吸着固定した多孔性炭素材料を用いる簡易型ビタミンCセンサーの開発(<特集>「若手研究者の初論文」)

概要

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The utilization of biological tissues containing large amount of enzymes is effective for fabricating inexpensive enzyme sensors, but the lifetime of enzymes in a biological tissue is rather short due to its putrefaction compared with the enzyme sensors using an immobilized purified enzyme membrane. In this work, cucumber juice containing ascorbte oxidase (ASOD) was used to immobilize ASOD to a porous carbon material by adsorption; this modified carbon material was combined with an oxygen electrode to fabricate a L-ascorbic acid (AsA) sensor. We found that an excellent linear relationship between AsA concentration and current response was obtained, and the stability of the sensor response was longer than at least one month. The reason why the lifetime of the enzyme sensor increases is that the microdebris of tissue is confined into the micropores of the carbon fibers, and the leaking or unfolding of enzyme does not take place. The porous carbon material adsorbed by purified ASOD also showed a similar long lifetime and a chemically amplified response (detection limit was 2.0×10^<-8>M) was observed by 0.05M dithiothreithol (DTT). However, in the case of a microtissue adsorbed carbon material, amplification of the sensor response using DTT was not observed, because the access of DTT to enzyme in tissue is difficult, differing from purified enzyme.
社団法人日本分析化学会の論文
2005-09-05